Comparison of real-time polymerase chain reaction with the COBAS Amplicor test for quantitation of hepatitis B virus DNA in serum samples
نویسندگان
چکیده
منابع مشابه
Comparison of branched DNA and real-time polymerase chain reaction methods in quantitation of hepatitis B virus DNA.
H B virus (HBV) is a major causative agent of chronic hepatitis, and can cause liver cirrhosis and hepatocellular carcinoma. Serological assays are not sufficient for the diagnosis of HBV infection. Measurement of HBV DNA levels are routinely used to identify infectious chronic carriers, and to predict and monitor the efficacies of antiviral treatment regiments.1 A number of commercial assays a...
متن کاملAn in-house real-time polymerase chain reaction: standardisation and comparison with the Cobas Amplicor HBV monitor and Cobas AmpliPrep/Cobas TaqMan HBV tests for the quantification of hepatitis B virus DNA.
This study aimed to standardise an in-house real-time polymerase chain reaction (rtPCR) to allow quantification of hepatitis B virus (HBV) DNA in serum or plasma samples, and to compare this method with two commercial assays, the Cobas Amplicor HBV monitor and the Cobas AmpliPrep/Cobas TaqMan HBV test. Samples from 397 patients from the state of São Paulo were analysed by all three methods. Fif...
متن کامل[Real-time polymerase chain reaction assay for hepatitis B virus DNA quantification].
INTRODUCTION viral DNA levels in serum samples are a useful marker to monitor the disease progression and the treatment response in patients with chronic hepatitis B. Commercial kits for this purpose are available, but they are considerably expensive. OBJECTIVES to evaluate the analytical performance of a real-time polymerase chain reaction (RT-PCR) assay for Hepatitis B virus DNA quantificat...
متن کاملRapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction.
AIM To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen. METHODS Hepatitis B viral DNA was isolated from HBV carriers' semen and sera using phenol extraction method and QIAamp DNA blood mini kit (Qiagen, Germany). HBV DNA was detected by conventional PCR and quantified by TaqMan technology-based re...
متن کاملQuantitation of hepatitis C virus using an in-house real-time reverse transcriptase polymerase chain reaction in plasma samples.
Even with the most advanced 3rd-generation assays, the serologic window period of hepatitis C virus (HCV) is approximately 74 days. HCV RNA detection would reduce the risk of transmission during this period. Furthermore, quantitation of HCV RNA is necessary for proper planning of treatment, monitoring disease progression, and assessing response to antiviral therapy. We have standardized an in-h...
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ژورنال
عنوان ژورنال: World Journal of Gastroenterology
سال: 2008
ISSN: 1007-9327
DOI: 10.3748/wjg.14.479